Recombinant Trypsin, MS Grade
CAS No. 9002-07-7
MS-grade recombinant trypsin enzyme
Ordering Information
Overview
Recombinant Trypsin MS Grade is an ultra-high purity trypsin optimized specifically for mass spectrometry applications, with minimized autolysis peptides and low chymotryptic side activity. It delivers complete and highly specific cleavage at Lys and Arg residues for reproducible tryptic digest performance in peptide mapping, MAM and bottom-up proteomics workflows. Its recombinant animal-component free origin eliminates the variability and contamination risks of porcine-derived preparations.
Key Benefits
Ultra-high purity optimized for mass spectrometry applications with minimal autolysis peptides and low chymotryptic side activity
High cleavage specificity at Lys and Arg residues generating well-defined peptide fragments for LC-MS/MS analysis
Recombinant and animal-component free, eliminates variability and contamination risks associated with porcine pancreas-derived trypsin
Consistent lot-to-lot digest performance supporting reproducible peptide map profiles across analytical batches
Compatible with standard in-solution and in-gel digestion protocols and common denaturing agents including urea and guanidinium chloride
Suitable for MAM workflows, peptide mapping, bottom-up proteomics and intact/subunit mass analysis sample preparation
Lyophilized format enables flexible reconstitution and long-term reagent stability
Available in 1 mg, 5 mg and 25 mg formats supporting method development through high-throughput routine analytical use
Product Specifications
| Grade | MS Grade |
| Enzyme Source | Recombinant; animal-component free |
| Specificity | Cleaves C-terminal to Lys and Arg residues; minimal chymotryptic activity |
| Form | Lyophilized Powder |
| Storage Condition | −15°C to −25°C |
| Optimal pH | 7.5–8.5 |
| Autolysis | Minimized; MS grade qualification includes autolysis peptide assessment |
| Compatibility | Urea, guanidinium chloride, RapiGest SF and standard digestion buffers |
| Purity | Defined by SDS-PAGE, activity assay and MS qualification |
