PNGase F FLASH®
CAS No. 83534-39-8
10-Minute Rapid Deglycosylation Enzyme for High-Throughput QC
Ordering Information
| Size / Format | Cat. No. | Description | Action |
|---|---|---|---|
| 50 RXN | PB-16-50RXN | PNGase F FLASH®, Liquid, 50 Reactions | Order |
Overview
PNGase F FLASH® is an engineered high-activity PNGase F variant designed for rapid and complete N-glycan removal in a fraction of the time required by conventional protocols. It is purpose-built for high-throughput QC release testing, multi-sample comparability studies and PAT environments where same-day sample preparation and analysis are operationally essential. Supplied as a ready-to-use liquid in a 50-reaction format.
Workflow
Key Benefits
Dramatically reduced deglycosylation time compared to conventional PNGase F enabling same-day sample preparation and analysis
Complete removal of all N-linked glycans including complex, high-mannose and hybrid-type structures
Supplied in a convenient 50-reaction format supporting high-throughput and multi-sample workflows
Eliminates glycoform-related mass heterogeneity for clean intact mass spectra and accurate molecular weight determination
Simplifies icIEF and CZE charge variant profiles by removing glycosylation-associated charge heterogeneity
Compatible with standard denaturing and native deglycosylation protocols
Liquid formulation, no reconstitution required; ready to use directly from storage
Animal-component free recombinant enzyme suitable for regulated analytical applications
Product Specifications
| Grade | Analytical Grade |
| Enzyme Source | Recombinant engineered PNGase F; animal-component free |
| Specificity | Cleaves all N-linked glycans between innermost GlcNAc and Asn |
| Form | Liquid |
| Storage Condition | 2°C to 8°C |
| Optimal pH | 7.0–8.5 |
| Reaction Time | Rapid; significantly reduced versus standard PNGase F protocols |
| Format | 50 reactions per kit |
| Purity | Defined by SDS-PAGE and activity assay; CoA provided per lot |
