Provinaseᵀᴹ (Endonuclease)
CAS No. 9025-65-4
GMP-grade endonuclease for host cell DNA removal
Ordering Information
Overview
Provinaseᵀᴹ is a recombinant non-specific endonuclease derived from Serratia marcescens, expressed in E. coli and manufactured to GMP grade standards. It hydrolyzes all forms of nucleic acids, single- and double-stranded DNA and RNA, via a Mg²⁺-dependent phosphodiester bond cleavage mechanism, reducing them to 3–5 nucleotide fragments. Provinaseᵀᴹ is widely used in biopharmaceutical manufacturing for host cell DNA (hcDNA) clearance, a critical step in meeting regulatory nucleic acid limits for injectable biologics.
Workflow
Key Benefits
GMP-grade recombinant endonuclease for regulatory-compliant hcDNA clearance
Effective against all nucleic acid forms (ssDNA, dsDNA, ssRNA, dsRNA)
Reduces host cell DNA to 3–5 nt oligomers, supporting regulatory limits
Animal-component free; suitable for downstream injectable and gene therapy products
Compatible with a broad range of bioprocessing buffers and pH conditions (pH 6–10)
Available in scalable SKUs (100KU to 5MU) for process development through commercial manufacturing
Product Specifications
| PURITY | ≥99% by SDS-PAGE |
| ENZYME SOURCE | Recombinant Serratia marcescens endonuclease expressed in E. coli |
| FORM | Frozen Liquid |
| RECOMMENDED STORAGE CONDITIONS | −15°C to −25°C |
| COFACTOR REQUIREMENT | Mg²⁺ (typically 1-2 mM MgCl₂) |
| OPTIMAL pH | 6-10 (broad range); peak activity ~pH 8 |
| EXPIRATION | Per CoA; avoid repeated freeze-thaw cycles |
