PNGase F
CAS No. 83534-39-8
High-Purity N-Glycan Removal Enzyme for mAb Characterization
Ordering Information
| Size / Format | Cat. No. | Description | Action |
|---|---|---|---|
| 500U | PB-06-500U | PNGase F, Liquid, 500 Units | Order |
Overview
PNGase F is a highly active amidase that releases all N-linked glycans from glycoproteins by cleaving between the innermost GlcNAc and asparagine residues. It is the standard enzyme for complete N-glycan removal prior to intact mass spectrometry, charge variant analysis and glycan profiling in mAb characterization workflows. Supplied as a stable ready-to-use liquid formulation.
Workflow
Key Benefits
Complete removal of all N-linked glycans including complex, high-mannose and hybrid type structures
Enables accurate intact mass determination by eliminating glycoform-related mass heterogeneity
Simplifies icIEF and CZE charge variant profiles by resolving glycosylation-linked charge variants
Compatible with standard denaturing and native deglycosylation protocols
Suitable for N-glycan release prior to HILIC-FLR and LC-MS glycan profiling
Animal-component free recombinant enzyme for use in regulated analytical workflows
Product Specifications
| Grade | MS Grade |
| Enzyme Source | Recombinant; animal-component free |
| Specificity | Cleaves all N-linked glycans between innermost GlcNAc and Asn |
| Form | Liquid |
| Storage Condition | 2°C to 8°C |
| Optimal pH | 7.0–8.5 |
| Reaction Conditions | Compatible with denaturing (SDS/BME) and native protocols |
| Purity | Defined by SDS-PAGE and activity assay; CoA provided per lot |
