Recombinant Trypsin-EDTA Solution for Cell Culture, GMP Grade
CAS No. 9002-07-7
Sterile ready-to-use recombinant trypsin with EDTA
Ordering Information
Overview
Recombinant Trypsin-EDTA Solution GMP Grade is a sterile ready-to-use liquid combining recombinant trypsin with EDTA for enhanced cell detachment efficiency in GMP mammalian cell culture. EDTA disrupts divalent cation-dependent adhesion synergistically with trypsin-mediated proteolytic cleavage, enabling complete cell dissociation at lower enzyme concentrations and shorter incubation times. It is a direct regulatory-compliant replacement for porcine trypsin-EDTA solutions across GMP cell culture operations.
Key Benefits
Ready-to-use sterile liquid formulation, no reconstitution or preparation required
Recombinant and animal-component free, eliminates adventitious agent and TSE/BSE risk associated with porcine trypsin-EDTA preparations
EDTA synergy enhances cell detachment efficiency by disrupting divalent cation-dependent adhesion prior to enzymatic cleavage
GMP-grade manufacture with full documentation including Certificate of Analysis and Certificate of Origin
Consistent lot-to-lot performance supporting reproducible cell dissociation across seed train and production culture operations
Compatible with standard GMP mammalian cell culture systems including CHO, HEK293, Vero and primary human cells
Supports xeno-free and serum-free cell culture process requirements for cell therapy and ATMP manufacturing
Available in 100 mL and 500 mL formats supporting laboratory-scale and manufacturing-scale GMP cell culture operations
Product Specifications
| Grade | GMP Grade |
| Enzyme Source | Recombinant trypsin; animal-component free |
| Formulation | Trypsin combined with EDTA in physiological buffer |
| Form | Sterile Liquid Solution |
| Storage Condition | −15°C to −25°C |
| Optimal pH | 7.0–7.6 |
| Trypsin Concentration | 0.05% |
| EDTA Concentration | 0.02% |
| Sterility | Sterile filtered; refer to CoA |
| Documentation | CoA, Certificate of Origin and GMP compliance documentation available |
