Recombinant Carboxypeptidase B, Sequencing Grade
CAS No. 9025-24-5
Sequencing grade rCPB for C-terminal peptide cleavage
Ordering Information
Overview
Sequencing Grade rCPB is a highly purified exopeptidase that removes C-terminal lysine and arginine residues from polypeptides with high specificity. It is used in mAb analytical workflows to eliminate C-terminal heterogeneity artifacts that confound charge variant profiling by icIEF and CZE. Its recombinant origin ensures consistent performance across characterization and comparability studies.
Key Benefits
Sequencing-grade purity for reliable, reproducible analytical results
Specifically removes C-terminal Lys and Arg residues from polypeptides
Eliminates C-terminal charge heterogeneity artifacts in icIEF and CZE charge variant profiling
Simplifies intact mass spectra and peptide maps by reducing isoform complexity
Recombinant, animal-component free and is suitable for regulated analytical workflows
Compatible with standard mAb digestion buffers used prior to mass spectrometry and charge variant analysis
Available in three SKUs (1 mg, 5 mg, 10 mg) to support method development through routine QC use
Product Specifications
| Grade | Sequencing Grade |
| Enzyme Source | Recombinant; animal-component free |
| Specificity | Cleaves C-terminal Lys and Arg residues |
| Form | Frozen Liquid |
| Storage Condition | −15°C to −25°C |
| Optimal pH | 7.5–9.0 |
| Cofactor | Zn²⁺ (metalloenzyme; avoid metal chelators) |
| Compatibility | Standard digestion buffers; avoid EDTA/EGTA |
| Purity | Defined by SDS-PAGE and activity assay; Certificate of Analysis provided |
